{"id":11372,"date":"2021-03-08T16:58:58","date_gmt":"2021-03-08T15:58:58","guid":{"rendered":"https:\/\/micrasys.com\/?p=11372"},"modified":"2023-09-13T14:36:26","modified_gmt":"2023-09-13T12:36:26","slug":"orca-lightning","status":"publish","type":"post","link":"https:\/\/micrasys.com\/en\/orca-lightning\/","title":{"rendered":"ORCA-Lightning"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"11372\" class=\"elementor elementor-11372\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6680414 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6680414\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4d84cfb\" data-id=\"4d84cfb\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2e8cc60 elementor-widget elementor-widget-shortcode\" data-id=\"2e8cc60\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-shortcode\"><nav aria-label=\"breadcrumbs\" class=\"rank-math-breadcrumb\"><p><span class=\"last\">Home<\/span><\/p><\/nav><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a39cdd8 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"a39cdd8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-544313a\" data-id=\"544313a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f352d60 elementor-widget elementor-widget-spacer\" data-id=\"f352d60\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! 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elementor - v3.19.0 - 05-02-2024 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/micrasys.com\/wp-content\/uploads\/2021\/03\/C14120_2_light-16x9-1-1024x576.jpg\" class=\"attachment-large size-large wp-image-11225\" alt=\"\" srcset=\"https:\/\/micrasys.com\/wp-content\/uploads\/2021\/03\/C14120_2_light-16x9-1-1024x576.jpg 1024w, https:\/\/micrasys.com\/wp-content\/uploads\/2021\/03\/C14120_2_light-16x9-1-300x169.jpg 300w, https:\/\/micrasys.com\/wp-content\/uploads\/2021\/03\/C14120_2_light-16x9-1-768x432.jpg 768w, https:\/\/micrasys.com\/wp-content\/uploads\/2021\/03\/C14120_2_light-16x9-1-1536x864.jpg 1536w, https:\/\/micrasys.com\/wp-content\/uploads\/2021\/03\/C14120_2_light-16x9-1-2048x1152.jpg 2048w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" title=\"\">\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-bfef2bc\" data-id=\"bfef2bc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9353ee9 text-wrap elementor-widget elementor-widget-heading\" data-id=\"9353ee9\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.19.0 - 05-02-2024 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">ORCA-Lightning Digital CMOS camera: C14120-20P<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-214bb61 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"214bb61\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5c4b67d\" data-id=\"5c4b67d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1e7954c text-wrap elementor-widget elementor-widget-text-editor\" data-id=\"1e7954c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.19.0 - 05-02-2024 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>For imaging scientists, inspiration is often found at the edges\u2014at the spatial, temporal, and optical boundaries of a system. It\u2019s that grainy image of the cytoskeleton that has just enough photons to confidently resolve two unique molecules. It\u2019s data extracted from a few pixels, whittled away from a larger array, to achieve capture rates relevant to neuronal signaling. It\u2019s the mesmerizing cellular migrations of a developing embryo, reconstructed from a lightsheet time-lapse, that offer new insights and spark new questions.<br \/>By more than doubling speed, resolution and field of view relative to current scientific cameras, the ORCA-Lightning is designed to inspire new frontiers of exploration. Compared to a Gen II sCMOS, the ORCA-Lightning delivers 2 times the pixel area, 2.8 times the pixels and 3.4 times faster pixel-per-second readout.<br \/>All these increases translate to the ability to capture more image data faster, and still achieve Hamamatsu-quality images and information content. So whether you\u2019re developing the next cutting-edge lightsheet system or building a library of the mouse connectome, the high throughput of the ORCA-Lightning will get you to your goal faster. And, we hope, open doors to new discoveries.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a13796c elementor-align-left elementor-widget__width-inherit elementor-widget elementor-widget-button\" data-id=\"a13796c\" data-element_type=\"widget\" id=\"spec\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"mailto:info@micrasys.com\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-text\">Request product<\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element 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(min-width:768px){.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tabs{display:flex}.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tabs-wrapper{flex-direction:column}.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tabs-content-wrapper{flex-grow:1;border-style:solid solid solid none}.elementor-widget-tabs.elementor-tabs-view-horizontal .elementor-tab-content{border-style:none solid solid}.elementor-widget-tabs.elementor-tabs-alignment-center .elementor-tabs-wrapper,.elementor-widget-tabs.elementor-tabs-alignment-end .elementor-tabs-wrapper,.elementor-widget-tabs.elementor-tabs-alignment-stretch .elementor-tabs-wrapper{display:flex}.elementor-widget-tabs.elementor-tabs-alignment-center .elementor-tabs-wrapper{justify-content:center}.elementor-widget-tabs.elementor-tabs-alignment-end .elementor-tabs-wrapper{justify-content:flex-end}.elementor-widget-tabs.elementor-tabs-alignment-stretch.elementor-tabs-view-horizontal .elementor-tab-title{width:100%}.elementor-widget-tabs.elementor-tabs-alignment-stretch.elementor-tabs-view-vertical .elementor-tab-title{height:100%}.elementor-tabs .elementor-tab-mobile-title{display:none}}<\/style>\t\t<div class=\"elementor-tabs\">\n\t\t\t<div class=\"elementor-tabs-wrapper\" role=\"tablist\" >\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-6751\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-6751\" aria-expanded=\"false\">Properties<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-6752\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-6752\" aria-expanded=\"false\">Specifications<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t<div class=\"elementor-tabs-content-wrapper\" role=\"tablist\" aria-orientation=\"vertical\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-6751\" aria-expanded=\"false\">Properties<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-6751\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-6751\" tabindex=\"0\" hidden=\"false\"><div class=\"Section Type--ModelModule\"><div class=\"Inner\"><h2>Features<\/h2><ul class=\"Gen-List List__Col Type--Cube\"><li>High resolution : 4608 x 2592 (12 Megapixels)<\/li><li>High Speed: 121 frames\/s (at full resolution)<\/li><li>Read noise : 2.0 electrons rms (Standard Full Well Capacity mode)<\/li><li>Pixel size : 5.5 \u03bcm X 5.5 \u03bcm<\/li><li>Dynamic Range 17 000:1 (High Full Well Capacity mode)<\/li><li>Peak QE : 60 % (at 550 nm)<\/li><\/ul><\/div><\/div><div class=\"Section\"><div class=\"Inner\"><h2><span class=\"Txt__Limit\">High-speed imaging<\/span><\/h2><div class=\"Gen-Gr-Txt\"><div class=\"Gen-Parts\"><p class=\"Gen-Txt\">Can even take high-speed images which were difficult to fully capture until now, with approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.<\/p><p>\u00a0<\/p><\/div><div class=\"Gen-Cols Cols__Row Type--Eq2\"><div class=\"Col\"><p class=\"Figure Figure__Image\"><img decoding=\"async\" src=\"https:\/\/www.hamamatsu.com\/blobs\/1328792147751?ssbinary=true\" alt=\"C14120-20P Comparison of speed\" width=\"768\" height=\"850\" title=\"\"><\/p><div class=\"Gen-Parts\"><p class=\"Gen-Txt Gen-Txt__Caption\">Speed comparison with ORCA-Flash4.0 V3<\/p><\/div><\/div><div class=\"Col Type_MT\"><p>Cameras used in light-sheet microscopes put emphasis on high speed, demanding fast readout. Because images with increased readout speed result in obtaining fewer photons upon capture, it is necessary to keep the camera&#8217;s readout noise low when trying to capture high S\/N images. ORCA-Lightning can capture images at approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.<\/p><p>Using CoaXPress as an interface and optimizing high speed data transfer to the PC, ORCA-Lightning realizes high-speed image acquisition.<\/p><\/div><\/div><\/div><div class=\"Gen-Gr-Header\"><div class=\"Gen-Header__03\"><h3>Readout speed (for each effective number of pixels and modes)<\/h3><\/div><\/div><div class=\"Gen-Parts\"><p class=\"Gen-Txt\">Customers can select the best mode depending on their usage.<br \/>In addition to a readout from a Standard Full Well Capacity mode (12 bit), ORCA-Lightning also supports a readout from a High Full Well Capacity mode (16 bit) for high dynamic range.<\/p><\/div><div class=\"Gen-Gr-Table\"><div class=\"Gen-table08\"><div class=\"Inner\"><div class=\"Wrapper Wrapper__Table\"><table class=\"Table Table__Common\"><colgroup> <col \/> <col \/> <col \/> <col \/> <col \/> <col \/> <col \/> <col \/> <col \/> <\/colgroup><thead><tr><th rowspan=\"3\">Effective number of pixels<br \/>(H) \u00d7 (V)<\/th><th colspan=\"8\">Readout speed (frames per second)<\/th><\/tr><tr><th colspan=\"2\">Free running mode *1<\/th><th colspan=\"2\">Edge trigger mode *2<\/th><th colspan=\"2\">Synchronous readout trigger mode *3<\/th><th colspan=\"2\">Global reset trigger mode *4<\/th><\/tr><tr><th>12 bit<\/th><th>16 bit<\/th><th>12 bit<\/th><th>16 bit<\/th><th>12 bit<\/th><th>16 bit<\/th><th>12 bit<\/th><th>16 bit<\/th><\/tr><\/thead><tbody><tr><th>4608 \u00d7 2592<\/th><td>121<\/td><td>30<\/td><td>121<\/td><td>30<\/td><td>121<\/td><td>30<\/td><td>120<\/td><td>30<\/td><\/tr><tr><th>2688 \u00d7 2592<\/th><td>208<\/td><td>30<\/td><td>207<\/td><td>30<\/td><td>208<\/td><td>30<\/td><td>207<\/td><td>30<\/td><\/tr><tr><th>2560 \u00d7 2560<\/th><td>221<\/td><td>30<\/td><td>220<\/td><td>30<\/td><td>221<\/td><td>30<\/td><td>220<\/td><td>30<\/td><\/tr><tr><th>2048 \u00d7 2048<\/th><td>307<\/td><td>38<\/td><td>305<\/td><td>38<\/td><td>308<\/td><td>38<\/td><td>305<\/td><td>38<\/td><\/tr><tr><th>1024 \u00d7 1024<\/th><td>610<\/td><td>76<\/td><td>603<\/td><td>75<\/td><td>612<\/td><td>76<\/td><td>600<\/td><td>75<\/td><\/tr><tr><th>512 \u00d7 512<\/th><td>1201<\/td><td>150<\/td><td>1175<\/td><td>146<\/td><td>1210<\/td><td>151<\/td><td>1166<\/td><td>145<\/td><\/tr><tr><th>256 \u00d7 256<\/th><td>2332<\/td><td>291<\/td><td>2234<\/td><td>279<\/td><td>2367<\/td><td>295<\/td><td>2203<\/td><td>275<\/td><\/tr><tr><th>128 \u00d7 4<\/th><td>31 725<\/td><td>3965<\/td><td>19 828<\/td><td>2478<\/td><td>39 657<\/td><td>4957<\/td><td>17 625<\/td><td>2203<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><div class=\"Gen-Parts\"><p class=\"Gen-Txt Gen-Txt__Caption\">*1 Free running mode is operated via camera alone.<br \/>*2 Edge trigger mode starts the exposure by the edge (rising\/falling edge) of the trigger signal input to the external device.<br \/>*3 Synchronous readout trigger mode ends the exposure of the camera and readout is started, while the next exposure is simultaneously started by the edge (rising\/falling edge) of the trigger signal input to the external device.<br \/>*4 Global reset trigger mode starts the exposure of all pixels all together by synchronizing the trigger signal from external device.<\/p><\/div><div class=\"Gen-Gr-Header\"><div class=\"Gen-Header__03\"><h3>High speed test of 3D imaging<\/h3><\/div><\/div><div class=\"Gen-Gr-Txt\"><div class=\"Gen-Cols Cols__Row Type--Eq2\"><div class=\"Col Type_MT\"><p>We took consecutive images and produced 3D imaging of a free-swimming Volvox.<br \/>When capturing the image of a free-swimming Volvox, imaging speed of 2-dimensional (area of observation) and 3-dimensional (number of images) settings were selected with a precondition of approximately 8 volumes per second.<br \/>With its high speed, ORCA-Lightning can capture clearer images within a wide range compared to ORCA-Flash4.0 V3.<\/p><p>Imaging conditions<\/p><ul class=\"Gen-List List__Col Type--Cube\"><li>Microscope: ezDSLM (light-sheet microscope)<\/li><li>Objective lens: 10\u00d7<\/li><li>Excitation wavelength: 488 nm<\/li><li>Fluorescence wavelength: red (autofluorescence)<\/li><li>Sample: Volvox<\/li><li>Observation target: Free-swimming Volvox<\/li><li>Method of imaging: Acquisition of approximately 8 volume image (stereoscopic) per second<\/li><\/ul><\/div><div class=\"Col\"><p class=\"Figure Figure__Image\"><img decoding=\"async\" src=\"https:\/\/www.hamamatsu.com\/blobs\/1328792147808?ssbinary=true\" alt=\"C14120-20P Comparison of 3D imaging\" width=\"768\" height=\"475\" title=\"\"><\/p><div class=\"Gen-Parts\"><p class=\"Gen-Txt Gen-Txt__Caption\">Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) \/ National Institute for Basic Biology (NIBB), Dr.Atsushi Taniguchi, Dr.Shigenori Nonaka<\/p><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"Section\"><div class=\"Inner\"><h2><span class=\"Txt__Limit\">Wide field of view<\/span><\/h2><div class=\"Gen-Gr-Txt\"><div class=\"Gen-Cols Cols__Row Type--Eq2\"><div class=\"Col\"><p class=\"Figure Figure__Image\"><img decoding=\"async\" src=\"https:\/\/www.hamamatsu.com\/blobs\/1328792147637?ssbinary=true\" alt=\"C14120-20P Comparison of sensor size\" width=\"768\" height=\"554\" title=\"\"><\/p><div class=\"Gen-Parts\"><p class=\"Gen-Txt Gen-Txt__Caption\">Comparison of sensor size between ORCA-Flash4.0 V3 and ORCA-Lightning<\/p><\/div><\/div><div class=\"Col Type_MT\"><p>ORCA-Lightning covers the twice large field of view compared to ORCA-Flash4.0 V3, so that it can improve throughput and efficiency of work.<\/p><\/div><\/div><\/div><div class=\"Gen-Gr-Txt\"><div class=\"Gen-Cols Cols__Row Type--Eq2\"><div class=\"Col Type_MT\"><div class=\"Gen-Parts\"><div class=\"Gen-Header__03 Type--NoSeparate\"><h3>Sample image 1: Test pattern<\/h3><\/div><\/div><div class=\"Gen-Media\"><p class=\"Figure Figure__Image\"><img decoding=\"async\" src=\"https:\/\/www.hamamatsu.com\/blobs\/1328792147694?ssbinary=true\" alt=\"C14120-20P, Comparison of sensor size, test pattern\" width=\"3201\" height=\"2451\" title=\"\"><\/p><\/div><p>Imaging condition<\/p><ul class=\"Gen-List List__Col Type--Cube\"><li>Microscope: Nikon Ti2-E<\/li><li>Objective lens: CFI Plan Apo Lambda 10\u00d7 \/ 0.45<\/li><li>F mount adapter: TI-BDTV2 +DS-F<\/li><li>Sample: Plotting objective Micrometer (Calibrated in 0.01 mm girds, 2 mm Line length)<\/li><\/ul><\/div><div class=\"Col Type_MT\"><div class=\"Gen-Parts\"><div class=\"Gen-Header__03 Type--NoSeparate\"><h3>Sample image 2: Dendritic spines of neuron<\/h3><\/div><\/div><div class=\"Gen-Media\"><p class=\"Figure Figure__Image\"><img decoding=\"async\" src=\"https:\/\/www.hamamatsu.com\/blobs\/1328792151262?ssbinary=true\" alt=\"C14120-20P Imaging sample\" width=\"768\" height=\"588\" title=\"\"><\/p><div class=\"Gen-Parts\"><p class=\"Gen-Txt Gen-Txt__Caption\">Collaborator: Department of Cellular Neurobiology Graduate School of Medicine the University of Tokyo, Dr.Yutaro Kashiwagi, Dr.Shigeo Okabe<\/p><\/div><\/div><p>Imaging condition<\/p><ul class=\"Gen-List List__Col Type--Cube\"><li>Microscope: Nikon Ti2-E<\/li><li>Objective lens: CFI Plan Apo Lambda 100\u00d7 oil \/ 1.45<\/li><li>F mount adapter: TI-BDTV2 +DS-F<\/li><li>Sample: GFP expressed mouse hippocampal neurons in dispersed cell culture (3 weeks culture)<\/li><li>Pixel number: 4608(H) \u00d7 2592(V)<\/li><li>Exposure time: 20 ms<\/li><\/ul><\/div><\/div><\/div><\/div><\/div><div class=\"Section\"><div class=\"Inner\"><h2><span class=\"Txt__Limit\">Movie gallery<\/span><\/h2><div class=\"Gen-Gr-Txt\"><div class=\"Gen-Movie__Modal\"><div class=\"Cols Cols__Row Type--Eq3\"><div class=\"Col\"><div class=\"Module Module__Media Gen-Movie_type2\"><p class=\"Figure Figure__Image\"><a class=\"mfp-youtube Gen-ModalMovie__Toggle\" href=\"https:\/\/www.youtube.com\/watch?v=EeDm9yh09rE\" target=\"_blank\" rel=\"noopener nofollow\"><img decoding=\"async\" src=\"https:\/\/i.ytimg.com\/vi\/EeDm9yh09rE\/mqdefault.jpg\" alt=\"\" title=\"\"><\/a><\/p><p class=\"Caption\">Free-swimming <em>Volvox carteri<\/em> scaned by ezDSLM (Digital Scanned Light-sheet Microscope)<\/p><\/div><\/div><div class=\"Col\"><div class=\"Module Module__Media Gen-Movie_type2\"><p class=\"Figure Figure__Image\"><a class=\"mfp-youtube Gen-ModalMovie__Toggle\" href=\"https:\/\/www.youtube.com\/watch?v=65mMq-ma5Ns\" target=\"_blank\" rel=\"noopener nofollow\"><img decoding=\"async\" src=\"https:\/\/i.ytimg.com\/vi\/65mMq-ma5Ns\/mqdefault.jpg\" alt=\"\" title=\"\"><\/a><\/p><p class=\"Caption\">Free-swimming <em>Paramecium bursaria<\/em> scaned by ezDSLM (Digital Scanned Light-sheet Microscope)<\/p><\/div><\/div><div class=\"Col\"><div class=\"Module Module__Media Gen-Movie_type2\"><p class=\"Figure Figure__Image\"><a class=\"mfp-youtube Gen-ModalMovie__Toggle\" href=\"https:\/\/www.youtube.com\/watch?v=FyeyTqcUrrk\" target=\"_blank\" rel=\"noopener nofollow\"><img decoding=\"async\" src=\"https:\/\/i.ytimg.com\/vi\/FyeyTqcUrrk\/mqdefault.jpg\" alt=\"\" title=\"\"><\/a><\/p><p class=\"Caption\">GFP expressing blood cells of <em>Oryzias latipes<\/em> scaned by ezDSLM (Digital Scanned Light-sheet Microscope)<\/p><\/div><\/div><\/div><div class=\"Gen-Gr-Txt\"><p class=\"Gen-Txt\">Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) \/ National Institute for Basic Biology (NIBB), Dr. Atsushi Taniguchi, Dr. Shigenori Nonaka<br \/>Sample provider: National Institute for Basic Biology (NIBB), Spectrography and BioImaging Facility, Joe Sakamoto Ph. D (NBRP Medaka)<\/p><\/div><\/div><\/div><\/div><\/div><div class=\"Section\"><div class=\"Inner\"><h2><span class=\"Txt__Limit\">PC recommendation<\/span><\/h2><div class=\"Gen-Gr-Txt\"><p class=\"Gen-Txt\">With the introduction of the ORCA-Lightning, users are now able to stream 12 megapixel images to their computers 121 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Lightning.<\/p><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-6752\" aria-expanded=\"false\">Specifications<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-6752\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-6752\" tabindex=\"0\" hidden=\"hidden\"><div class=\"Section\"><div class=\"Inner\"><h2><span class=\"Txt__Limit\">Specifications<\/span><\/h2><div class=\"Wrapper Wrapper__Table JS__Scroll mCustomScrollbar _mCS_1 mCS_no_scrollbar\"><div id=\"mCSB_1\" class=\"mCustomScrollBox mCS-hpk mCSB_horizontal mCSB_outside\" tabindex=\"0\"><div id=\"mCSB_1_container\" class=\"mCSB_container mCS_x_hidden mCS_no_scrollbar_x\" dir=\"ltr\"><table class=\"detail_table Table__sameFontSize\"><tbody><tr><th>Type number<\/th><td>C14120-20P<\/td><\/tr><tr><th>Quantum efficency<\/th><td>Over 60 % (at 550 nm)<\/td><\/tr><tr><th>Imaging device<\/th><td>Scientific CMOS image sensor<\/td><\/tr><tr><th>Effective no. of pixels<\/th><td>4608 (H) \u00d7 2592 (V)<\/td><\/tr><tr><th>Cell size<\/th><td>5.5 \u03bcm (H) \u00d7 5.5 \u03bcm (V)<\/td><\/tr><tr><th>Effective area<\/th><td>25.344 mm (H) \u00d7 14.256 mm (V)<\/td><\/tr><tr><th>Full well capacity<\/th><td>Standard Full Well Capacity mode: 1000 electrons<br \/>High Full Well Capacity mode: 38 000 electrons<\/td><\/tr><tr><th>Readout speed<\/th><td>121 frames\/s (at full resolution)<\/td><\/tr><tr><th>Readout noise<\/th><td>Standard Full Well Capacity mode\uff1a1.5 electrons median<br \/>Standard Full Well Capacity mode\uff1a2.0 electrons rms<br \/>High Full Well Capacity mode\uff1a2.2 electrons median<br \/>High Full Well Capacity mode: 2.7 electrons rms<\/td><\/tr><tr><th>Exposure time<\/th><td>Standard Full Well Capacity mode: 6.304 \u03bcs to 1 s<br \/>High Full Well Capacity mode: 50.432 \u03bcs to 1 s<\/td><\/tr><tr><th>Cooling temperature<\/th><td>Forced-air cooled (Ambient temperature: +25 \u02daC): +20 \u02daC<br \/>Water cooled: +20 \u02daC<\/td><\/tr><tr><th>Dark current<\/th><td>15 electrons\/pixel\/s (Typ.) : Air cooled (cooling temperature: +20 \u02daC)<\/td><\/tr><tr><th>Dynamic range<\/th><td>Standard Full Well Capacity mode: 650 : 1<br \/>High Full Well Capacity mode: 17 000 : 1<\/td><\/tr><tr><th>Sub-array<\/th><td>Yes<\/td><\/tr><tr><th>Binning<\/th><td>2\u00d72, 4\u00d74<\/td><\/tr><tr><th>External trigger input<\/th><td>Edge trigger, Global reset edge trigger, Level trigger, Global reset level trigger, Synchronous readout trigger, Start trigger<\/td><\/tr><tr><th>External trigger signal routing<\/th><td>SMA<\/td><\/tr><tr><th>Trigger delay function<\/th><td>0 s to 10 s in 1 \u03bcs steps<\/td><\/tr><tr><th>Interface<\/th><td>CoaXPress (Quad CXP-6)<\/td><\/tr><tr><th>A\/D converter<\/th><td>Standard Full Well Capacity mode: 12 bit<br \/>High Full Well Capacity mode: 16 bit<\/td><\/tr><tr><th>Power consumption<\/th><td>Approx. 200 VA<\/td><\/tr><tr><th>Ambient operating temperature<\/th><td>0 \u02daC to +40 \u02daC : Forced-air cooled (With no condensation)<\/td><\/tr><tr><th>Ambient operating humidity<\/th><td>30 % to 80 %<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><div class=\"Section Type--ModelModule\"><div class=\"Inner\"><h2>Spectral response<\/h2><div class=\"Cols Cols__Row Type--Eq2\"><div class=\"Col\"><div class=\"Module Module__Media\"><p class=\"Figure Figure__Image\"><img decoding=\"async\" src=\"https:\/\/www.hamamatsu.com\/blobs\/1328791634124?blobheadername1=content-disposition&amp;blobheadervalue1=inline%3Bfilename%3Ds_c14120-20p_sr_en.jpg&amp;ssbinary=true\" alt=\"c14120-20p Spectral response\" title=\"\"><\/p><\/div><\/div><div class=\"Col\"><div class=\"Module Module__Media\"><p class=\"Figure Figure__Image\">\u00a0<\/p><\/div><\/div><\/div><\/div><\/div><div class=\"Section Type--ModelModule\"><div class=\"Inner\"><h2>Dimensions<\/h2><div class=\"Cols Cols__Row Type--Eq2\"><div class=\"Col\"><div class=\"Module Module__Media\"><p class=\"Figure Figure__Image\"><img decoding=\"async\" src=\"https:\/\/www.hamamatsu.com\/blobs\/1328791634065?blobheadername1=content-disposition&amp;blobheadervalue1=inline%3Bfilename%3Ds_c14120-20p_do_en.jpg&amp;ssbinary=true\" alt=\"c14120-20p dimensional outline\" title=\"\"><\/p><\/div><\/div><div class=\"Col\">\u00a0<\/div><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ded45b8 elementor-section-full_width 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It\u2019s that grainy image of the cytoskeleton that has just enough photons to confidently resolve two unique molecules. 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