ORCA-Lightning Digital CMOS Kamera: C14120-20P
Für Wissenschaftler im Bereich der Bildgebung findet sich Inspiration häufig an den Rändern – an den räumlichen, zeitlichen und optischen Grenzen eines Systems. Es ist dieses körnige Bild des Zytoskeletts, das gerade genug Photonen enthält, um zwei einzigartige Moleküle sicher aufzulösen. Daten, die aus wenigen Pixeln extrahiert und von einem größeren Array entfernt wurden, um für die neuronale Signalübertragung relevante Erfassungsraten zu erzielen. Es sind die faszinierenden zellulären Wanderungen eines sich entwickelnden Embryos, die aus einem Zeitraffer eines Light Sheets rekonstruiert wurden und neue Erkenntnisse bieten und neue Fragen aufwerfen.
Durch die Verdoppelung von Geschwindigkeit, Auflösung und Sichtfeld im Vergleich zu aktuellen wissenschaftlichen Kameras, soll die ORCA-Lightning neue Grenzen der Erforschung eröffnen. Im Vergleich zur sCMOS der Generation II liefert die ORCA-Lightning die 2-fache Pixelfläche, 2,8-fache Pixelanzahl und die 3,4-fach schnellere Pixel-pro-Sekunde-Anzeige.
All diese Verbesserungen führen dazu, dass mehr Bilddaten schneller erfasst werden können und dennoch Bilder und Informationsinhalte in Hamamatsu-Qualität erzielt werden können. Egal, ob Sie das nächste hochmoderne Lightsheet-System entwickeln oder eine Bibliothek des Maus-Connectoms erstellen, der hohe Durchsatz der ORCA-Lightning bringt Sie schneller zum Ziel. Und wir hoffen, es werden Türen für neue Entdeckungen geöffnet.
Features
- High resolution : 4608 x 2592 (12 Megapixels)
- High Speed: 121 frames/s (at full resolution)
- Read noise : 2.0 electrons rms (Standard Full Well Capacity mode)
- Pixel size : 5.5 μm X 5.5 μm
- Dynamic Range 17 000:1 (High Full Well Capacity mode)
- Peak QE : 60 % (at 550 nm)
High-speed imaging
Can even take high-speed images which were difficult to fully capture until now, with approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.
Speed comparison with ORCA-Flash4.0 V3
Cameras used in light-sheet microscopes put emphasis on high speed, demanding fast readout. Because images with increased readout speed result in obtaining fewer photons upon capture, it is necessary to keep the camera’s readout noise low when trying to capture high S/N images. ORCA-Lightning can capture images at approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.
Using CoaXPress as an interface and optimizing high speed data transfer to the PC, ORCA-Lightning realizes high-speed image acquisition.
Readout speed (for each effective number of pixels and modes)
Customers can select the best mode depending on their usage.
In addition to a readout from a Standard Full Well Capacity mode (12 bit), ORCA-Lightning also supports a readout from a High Full Well Capacity mode (16 bit) for high dynamic range.
| Effective number of pixels (H) × (V) |
Readout speed (frames per second) | |||||||
|---|---|---|---|---|---|---|---|---|
| Free running mode *1 | Edge trigger mode *2 | Synchronous readout trigger mode *3 | Global reset trigger mode *4 | |||||
| 12 bit | 16 bit | 12 bit | 16 bit | 12 bit | 16 bit | 12 bit | 16 bit | |
| 4608 × 2592 | 121 | 30 | 121 | 30 | 121 | 30 | 120 | 30 |
| 2688 × 2592 | 208 | 30 | 207 | 30 | 208 | 30 | 207 | 30 |
| 2560 × 2560 | 221 | 30 | 220 | 30 | 221 | 30 | 220 | 30 |
| 2048 × 2048 | 307 | 38 | 305 | 38 | 308 | 38 | 305 | 38 |
| 1024 × 1024 | 610 | 76 | 603 | 75 | 612 | 76 | 600 | 75 |
| 512 × 512 | 1201 | 150 | 1175 | 146 | 1210 | 151 | 1166 | 145 |
| 256 × 256 | 2332 | 291 | 2234 | 279 | 2367 | 295 | 2203 | 275 |
| 128 × 4 | 31 725 | 3965 | 19 828 | 2478 | 39 657 | 4957 | 17 625 | 2203 |
*1 Free running mode is operated via camera alone.
*2 Edge trigger mode starts the exposure by the edge (rising/falling edge) of the trigger signal input to the external device.
*3 Synchronous readout trigger mode ends the exposure of the camera and readout is started, while the next exposure is simultaneously started by the edge (rising/falling edge) of the trigger signal input to the external device.
*4 Global reset trigger mode starts the exposure of all pixels all together by synchronizing the trigger signal from external device.
High speed test of 3D imaging
We took consecutive images and produced 3D imaging of a free-swimming Volvox.
When capturing the image of a free-swimming Volvox, imaging speed of 2-dimensional (area of observation) and 3-dimensional (number of images) settings were selected with a precondition of approximately 8 volumes per second.
With its high speed, ORCA-Lightning can capture clearer images within a wide range compared to ORCA-Flash4.0 V3.
Imaging conditions
- Microscope: ezDSLM (light-sheet microscope)
- Objective lens: 10×
- Excitation wavelength: 488 nm
- Fluorescence wavelength: red (autofluorescence)
- Sample: Volvox
- Observation target: Free-swimming Volvox
- Method of imaging: Acquisition of approximately 8 volume image (stereoscopic) per second
Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) / National Institute for Basic Biology (NIBB), Dr.Atsushi Taniguchi, Dr.Shigenori Nonaka
Wide field of view
Comparison of sensor size between ORCA-Flash4.0 V3 and ORCA-Lightning
ORCA-Lightning covers the twice large field of view compared to ORCA-Flash4.0 V3, so that it can improve throughput and efficiency of work.
Sample image 1: Test pattern
Imaging condition
- Microscope: Nikon Ti2-E
- Objective lens: CFI Plan Apo Lambda 10× / 0.45
- F mount adapter: TI-BDTV2 +DS-F
- Sample: Plotting objective Micrometer (Calibrated in 0.01 mm girds, 2 mm Line length)
Sample image 2: Dendritic spines of neuron
Collaborator: Department of Cellular Neurobiology Graduate School of Medicine the University of Tokyo, Dr.Yutaro Kashiwagi, Dr.Shigeo Okabe
Imaging condition
- Microscope: Nikon Ti2-E
- Objective lens: CFI Plan Apo Lambda 100× oil / 1.45
- F mount adapter: TI-BDTV2 +DS-F
- Sample: GFP expressed mouse hippocampal neurons in dispersed cell culture (3 weeks culture)
- Pixel number: 4608(H) × 2592(V)
- Exposure time: 20 ms
Movie gallery
Free-swimming Volvox carteri scaned by ezDSLM (Digital Scanned Light-sheet Microscope)
Free-swimming Paramecium bursaria scaned by ezDSLM (Digital Scanned Light-sheet Microscope)
GFP expressing blood cells of Oryzias latipes scaned by ezDSLM (Digital Scanned Light-sheet Microscope)
Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) / National Institute for Basic Biology (NIBB), Dr. Atsushi Taniguchi, Dr. Shigenori Nonaka
Sample provider: National Institute for Basic Biology (NIBB), Spectrography and BioImaging Facility, Joe Sakamoto Ph. D (NBRP Medaka)
PC recommendation
With the introduction of the ORCA-Lightning, users are now able to stream 12 megapixel images to their computers 121 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Lightning.
Spezifikationen
Spectral response
Dimensions
