Ultra-Stable US-2000 Flat-Top XY Automated Stage for Super Resolution Microscopy
The new US-2000 by ASIimaging is a motorized stage designed to hold really still. This boils down to moving as little as possible with temperature changes.
The new US-2000 should never be used for scanning, and repeatability is greatly sacrificed for thermal stability. The most prominent application of the US-2000 single-molecule localization microscopy (SMLM), a category of super-resolution microscopy which includes PALM, STORM, DNA-PAINT, etc.
SMLM uses long exposure times to image individual stationary fluorophores. During post-processing, the centre of each point is determined with an accuracy/resolution of up to 10 nanometres, which is why the object stage must be kept very still.
The US-2000 was designed to mount to a RAMM frame or stand-alone.
It has been adapted to fit on Nikon Ti2 and Olympus IX71/73/81/83. No option currently exists for Zeiss or Leica stands.
US-2000 Features
- Closed loop DC servo control of the X, Y, and Z-axes for precise positioning and highly repeatable focusing
- Wide dynamic speed range with XY joystick
- Utilizes ASI’s proven Z-Axis drives
- Z-axis clutch for easy switching between manual and motor driven focus control
- Backlit LCD display shows X, Y, and Z coordinates
- “Zero” and “Home” buttons for simple stand-alone operations
- Compact, ergonomic tabletop control unit size is 6”D x 9”W x 3”H (9 x 23 x 16.5 cm)
- Microprocessor control with RS-232 serial and USB communications
- Travel Range will scan full well plate in most circumstances
- Proven operation with many popular software packages
US-2000 Options
- Piezo top plates with Z ranges of 150, 300 and 500 µm
- Larger stage top plate for attachment of micromanipulators, microinjectors, etc…
- Stage wings for even more room for attachments
- Auto-focus for stages with ASI Z-axis drives (requires NTSC, PAL, or S-Video analog signal)
- Other lead screw pitches are available
US-2000 Lead Screw Options
| Lead Screw Pitch Options | Rotary Encoder Resolution | Maximum Speed |
| 25.40 mm (Ultra-coarse) | 88 nm | 28 mm/sec |
| 12.70 mm (Super-coarse) | 44 nm | 14 mm/sec |
| 6.35 mm (Standard) | 22 nm | 7 mm/sec |
| 1.59 mm (Fine) | 5.5 nm | 1.75 mm/sec |
| 0.635 mm (Extra-fine) | 2.2 nm | 0.7 mm/sec |
US-2000 Specifications for Standard Configuration
| XY axis range of travel | 50 mm x 50 mm up to 120 mm x 75 mm |
| XY axis resolution (encoder step) | 2.5 nm (10 nm at optical axis) |
| XY axis RMS repeatability | < 200 nm |
| XY axis maximum velocity (dependent on lead screw pitch option) | 0.635 mm/sec |
| Z axis resolution with optional piezo top plate | 2.2 nm with 16 bit DAC 1.1 nm with 17 bit DAC |
| Z axis repeatability with optional piezo top plate | +/- 2 nm |
| Z axis maximum velocity with optional piezo top plate | ~ 10 ms for most moves |
| Max recommended load | 1 kg |
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